Menu
CINN
  • HOME
  • WHO ARE WE
    • Overview
    • Facts & figures
    • Our industrial partners
    • Team
  • WHAT WE DO
    • Research
    • Application fields
    • Scientific & technical services
    • Communication & outreach
  • CINN IN PROGRESS
    • Projects
    • Publications
    • Blogs
    • News
  • KNOWLEDGE DATABASE
    • Institutional/corporate info
    • Cultural Heritage
    • Health
    • Advanced Materials
    • ICT
    • Big science
  • CONTACT US
  • EnglishEnglish
    • EnglishEnglish
    • EspañolEspañol
Close Menu
1 January 2019

Identification of therapeutic targets in colon cancer through epigenetic edition

AdminCinn Projects Nanomedicine

Abstract

In recent years, the analysis of genome-wide DNA methylation patterns has made it possible to identify new biomarkers in colorectal cancer (CRC). Even though many of these alterations can be useful in the diagnosis, prognosis or even evaluation of response to treatment, their value as possible therapeutic targets has proven to be much more limited, mainly because their true role as causal or passenger perturbations in the tumoral process has not been determined. In order to identify aberrant DNA methylation patterns that can constitute possible biological
targets in CRC, we propose a novel experimental design based in a progressive screening of candidate genes and regions. Firstly, we will integrate epigenomic and transcriptomic data of ~30 normal and ~250 tumor tissue samples obtained from colon adenocarcinoma. Secondly, to determine true functional associations between changes in DNA methylation and gene expression, we will ectopically modify the levels of methylation by using CRISPR technology (fusion proteins of deactivated Cas9 with the catalytic domains of TET1, DEMETER and DNMT3) in tumoral cell lines, organoids, and animal models. Finally, to determine the clinical implications of the functional methylation alterations, we will analyze the methylation state and expression of candidate genes in 150
samples obtained from patients with colorectal tumors for which there is ample clinical information the disease progression and other factors. The obtained results may be used to the development of biomarkers based on functional aspects, and, more importantly, they will enable the identification of new therapeutic targets in colorectal cancer.

Project Details

Project Code: PI18/01527
Duration: 01/01/2019-31/12/2021
Funding: 196.020,00 €

Funding Instituto de Salud Carlos III

logo ISCIII
New reactivity ways of coordinated N-heterocycles to metal fragments ECOPLACKAGING-Vegetal fibres-reinforced PLA antimicrobial composites for packaging applications

Related Posts

enfermedad Huntington (1)

Projects

Identification of alterations in the subcellular architecture of peripheral tissues in Huntington’s disease

barandilla

Projects

Eradication of indirect contact transmission of SARS-CoV-2 by developing antimicrobial surfaces with inorganic antimicrobial additives

coronavirus

Projects

Design of molecular targets for rapid and efficient identification of SARS-CoV-2 RNA species in CoVid-19 patients

Back To Top
CINN

HOME

WHO ARE WE

> Overview
> Facts & figure
> Our industrial partners
> Team

WHAT WE DO

RESEARCH

> Modelling and simulation
> Nanostructured hybrid systems
> Synthesis of nanocomposites and bioinspired materials
> Nanomedicine

APPLICATION FIELDS

Health

  • Epigenetics
  • Inorganic antimicrobials
  • Periodontal and Peri Implant Diseases
  • Bone Grafts
  • Dental cad-cam materials

BIG SCIENCE

> Heat sinks
> Ir windows

ADVANCED MATERIALS

> Nanocomposite Powders
> EDM Ceramic
> Ultrastable ceramics
> Ultrahard metal-ceramic composites
> Graphene

TIC

> Quantum electronics
> Information storage
> Functional ceramics

SCIENTIFIC & TECHNICAL SERVICES

Nanotechnology Unit
Services

  • Biocompatibility Studies
  • Electromagnetic Characterization
  • Innovation
  • Mechanical Characterization
  • Microbiological Studies
  • Samples Preparation
  • Thermal Treatments and Sintering

COMMUNICATION & OUTREACH

CINN IN PROGRESS

> Projects
> Publications
> Blogs
> News

KNOWLEDGE DATABASE

> Institutional/Corporate info
> Heritage
> Health
> Advanced materials
> TIC
> Big science

CONTACT US

LEGAL ADVICE COOKIES