Our research is focused on the in situ analysis of the subcellular architecture and the characterization of the alterations in neurodegenerative diseases. Our working hypothesis is that the study of such alterations may contribute to gain insights into the pathophysiological basis of diseases, serve as a biomarker and enable the identification of new therapeutic targets. To this end, we have developed protocols based on cryo-preservation of tissue, we use leading-edge nanotomography imaging techniques and advanced three-dimensional (3D) image processing methods and artificial intelligence as instrumental tools to derive quantitative information.